| Primer | Species | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1061 | Holdemania filiformis | GCGATGGTGCATTACAGCC | TACTGTACCGTTGCGCACC | 59.64 | 60.37 | 291 | 46 |
100.00%
|
50.00%
|
| Primer 1062 | Holdemania filiformis | TCAAGAAGCGCATCGGCA | TCCGCACTCAATTCCGGAG | 60.05 | 59.78 | 202 | 46 |
100.00%
|
50.00%
|
| Primer 1063 | Holdemania filiformis | AGCCGAACGGTTTGTGGA | ACGCTTTGCAATGCAGCC | 59.49 | 60.05 | 188 | 46 |
100.00%
|
50.00%
|
| Primer 1064 | Holdemania filiformis | TATCAGCCGTTGGACGCA | AAGTCCGTAAGCAGCGCA | 59.03 | 59.66 | 265 | 46 |
100.00%
|
50.00%
|
| Primer 1065 | Holdemania filiformis | TCAAGGCAAGGTGCTGCA | ACGGATCTGACAGCGCAA | 59.80 | 59.34 | 199 | 45 |
100.00%
|
50.00%
|
| Primer 1066 | Holdemania filiformis | ACCTGCTGGTCACGCAAT | GCGATGATCAGGCCTCGTT | 59.57 | 60.23 | 207 | 45 |
100.00%
|
50.00%
|
| Primer 1067 | Holdemania filiformis | ACATCGTGGAAGAGCTGGC | TAAGCCGATCGTGCCGTT | 60.08 | 59.43 | 164 | 45 |
100.00%
|
50.00%
|
| Primer 1068 | Holdemania filiformis | TACGGCAGCGTTCCAGTT | AATGAGTCCGCAGCTGCT | 59.26 | 59.33 | 186 | 45 |
100.00%
|
50.00%
|
| Primer 1069 | Holdemania filiformis | TCAAGCGTTCGCTGGGTT | AATACACGTCGCTGCGGA | 59.89 | 59.43 | 165 | 45 |
100.00%
|
50.00%
|
| Primer 1070 | Holdemania filiformis | TGGTGCCGATCAAGCGTT | ATACACGTCGCTGCGGAT | 59.97 | 59.19 | 173 | 45 |
100.00%
|
50.00%
|